Jagadish soon learns that the bomber is a mere executor, a sleeper agent, whose only role was to plant the bomb. Jagadish captures the bomber again, and also forces the police officer who helped the bomber's escape, to commit suicide. He manages to capture the man who laid the bomb, but he escapes from the hospital where he was kept under custody. One day, Jagadish witnesses the explosion of a bus in which he and his police officer-friend Balaji had travelled. Nisha reciprocates after refusing him initially. Jagadish notices her in a boxing match and falls in love with her instantly. On the contrary, Nisha is a university-level boxer, who is completely modern in her outlook. At the bride-viewing ceremony, Jagadish makes up excuses to avoid marrying her, which includes commenting about her being old-fashioned.
On his arrival, his parents and younger sisters force him to see Nisha, who they choose for him to be married to. Jagadish Dhanapal, an intelligence officer in the Indian Army, returns to Mumbai from Kashmir. In 2014, Murugadoss remade the film in Hindi as Holiday: A Soldier Is Never Off Duty, while Baba Yadav remade it in Bengali as Game. However, it won four SIIMA Awards, six Vijay Awards, three Edison Awards, a Chennai Times Film Award, Ananda Vikatan Cinema Award, CineMAA Award each. A recipient of various accolades, the film went on to receive seven Filmfare Awards South nominations in the Tamil branch, but did not win in any category. Nevertheless, it received positive reviews and became a major commercial success, grossing over ₹0.001 billion (US$13,000) within 11 days of its release. The film went through numerous controversies, including protests from Islamic fringe groups, due to the objectionable portrayal of the Islamic community in the film. Thuppakki was released on 13 November 2012, Diwali day. The film's music was composed by Harris Jayaraj with cinematography handled primarily by Santosh Sivan and editing by A. Filming mostly took place in Mumbai, except for two song sequences which were taken in Bangkok and Switzerland respectively. Principal photography of the film commenced in January 2012 and ended in October 2012. The project, which marked the beginning of a collaboration between Vijay and Murugadoss, was reported in July 2011, and the film's title was formally announced on the same year in December. It revolves around an intelligence officer in the Indian Army on a mission to track down, destroy and deactivate a sleeper cell, after witnessing and barely escaping a bomb blast executed by the cell. Thanu under his studio V Creations, and distributed by Gemini Film Circuit, the film stars Vijay and Kajal Aggarwal, with Sathyan, Vidyut Jammwal, Jayaram, Manobala and Zakir Hussain in supporting roles. Gun) is a 2012 Indian Tamil-language action thriller film written and directed by AR Murugadoss. Our results provide mechanistic insights into how the novel rDNA transcription repressor BEND3 acts together with NoRC to actively coordinate the establishment of rDNA silencing.Thuppakki ( transl. BEND3 associates with the nucleolar-remodeling complex (NoRC), and SUMOylated BEND3 stabilizes NoRC component TTF-1-interacting protein 5 via association with ubiquitin specific protease 21 (USP21) debiquitinase. Loss of BEND3 increases histone H3K4 trimethylation and, correspondingly, decreases rDNA promoter DNA methylation, consistent with a role for BEND3 in rDNA silencing. We demonstrate in mammalian cells that BANP, E5R, and Nac1 (BEN) domain 3 (BEND3), a quadruple BEN domain-containing protein, localizes in nucleoli and binds to ribosomal RNA gene promoters to help repress rRNA genes. Whereas oncogenic agents stimulate rRNA gene transcription, tumor suppressors decrease rRNA gene transcription. rDNA silencing is one such mechanism that ensures the inactivity and hence the maintenance of a silenced state of a subset of rRNA gene copies. Several mechanisms establish and maintain transcriptional output from eukaryotic ribosomal DNA (rDNA) loci. Ribosome biogenesis dictates the translational capacity of cells.
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